Pancreatic Islet Embedding for Paraffin Sections

Multiparameter immunofluorescence on paraffin-embedded tissue sections.

Immunohistochemical techniques have gained increasing importance in diagnostics and research. While formalin-fixed, paraffin-embedded human tissue retains excellent morphology, the detection of antigens by immunofluorescence in its sections and especially the demonstration of multiple simultaneous antibodies have limitations. Double immunofluorescence labeling of routinely processed paraffin se...

Storage of Frozen Sections in Paraffin Blocks.

The development of the techniques for cutting frozen sections, in particular the use of cryostat microtomes (Pearse, 1960; Cunningham, Bitensky, Chayen, and Silcox, 1960 and 1962; Goodbody, 1963 a and b; Cunningham, 1963), has resulted in sections which not only are produced routinely very rapidly but which are completely acceptable histologically. Such sections would be ideal, especially where...

Lectin binding to formalin-fixed paraffin sections.

Lectins are potentially useful tools in histopathology for the identification of carbohydrates and distinguishing cells according to their type, differentiation or function. Conjugated to fluorescent or enzyme labels, lectins are simple to use on fresh tissue but fixation and processing sequesters glycoconjugates and dissolves out fat-linked sugars. We describe here the use of labelled antibodi...

A Rapid Method of Paraffin Embedding

Agar-gelatin for embedding tissues prior to paraffin processing.

Benchmarks Fixed tissues often need to be arranged in a particular configuration , order, or orientation prior to processing for paraffin embedding. Although it is possible to arrange tissues after infiltration (during the final stages of paraffin embedding), it can be more convenient and practical to do this at the time of dissection or tissue collection. Agar has been the classical medium use...